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KMID : 0545120100200030615
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Journal of Microbiology and Biotechnology
2010 Volume.20 No. 3 p.615 ~ p.621
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Hydrogenotrophic sulfate reduction in a gas-lift bioreactor operated at 9oC
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Nevatalo Laura M.
Bijmans Martijn F. M.
Lens Piet N. L.
Kaksonen Anna H.
Puhakka Jaakko A.
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Abstract
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The viability of low temperature sulfate reduction with hydrogen as electron donor was studied with a bench-scale gas-lift bioreactor (GLB) operated at 9oC. Prior to the GLB experiment, the temperature range of sulfate reduction of the inoculum was assayed. The results of the temperature gradient assay indicated that the inoculum was a psychrotolerant mesophilic enrichment culture that had an optimal temperature for sulfate reduction of 31oC, and minimum and maximum temperatures of 7oC and 41oC, respectively. In the GLB experiment at 9oC, a sulfate reduction rate of 500-600 mg L-1 d-1, corresponding to a specific activity of 173 mg SO42- g VSS-1 d-1, was obtained. The electron flow from the consumed H2-gas to sulfate reduction varied between 27 and 52 %, while the electron flow to acetate production decreased steadily from 15 % to 5 %. No methane was produced. Acetate was produced from CO2 and H2 by homoacetogenic bacteria. Acetate supported the growth of some heterotrophic sulfate-reducing bacteria. The sulfate reduction rate in the GLB was limited by the slow biomass growth rate at 9oC and low biomass retention in the reactor. Nevertheless, this study demonstrated the potential sulfate reduction rate of psychrotolerant sulfate-reducing mesophiles at sub-optimal temperature.
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KEYWORD
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Sulfate reduction, low temperature, gas-lift bioreactor, hydrogenotroph, homoacetogenesis
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